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human melanocyte growth supplement-2 (hmgs-2  (Thermo Fisher)


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    Thermo Fisher human melanocyte growth supplement-2 (hmgs-2
    Human Melanocyte Growth Supplement 2 (Hmgs 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human melanocyte growth supplement-2 (hmgs-2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    human melanocyte growth supplement-2 (hmgs-2 - by Bioz Stars, 2026-02
    90/100 stars

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    CCK-8 assay confirmed UCL, UCL-NC, and UCL-211 were not cytotoxic to NHM (a) and HDF (b) . c. Histogram for APC intensity comparison of UCL-211 uptake in NHM, HDF and HEK (corresponding to miR211-5p conjugated with Cy5). d. Uptake of UCLs in NHM, HDF and HEK-FITC intensity (from F-DOTAP in UCLs). e. Uptake of UCLs in NHM, HDF and HEK-APC intensity (from Cy5 conjugated on miR211-5p). f. Pigmentation effect on uptake in light-pigmented (LP), medium pigmented (MP), and dark-pigmented (DP) melanocytes cultured in <t>HMGS2</t> media. Data are represented as mean ± SD. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns-not significant). Biological replicates ( N = 2) and technical replicates ( n = 3).
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    human melanocyte growth supplement-2 (hmgs-2)  (Thermo Fisher)
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    CCK-8 assay confirmed UCL, UCL-NC, and UCL-211 were not cytotoxic to NHM (a) and HDF (b) . c. Histogram for APC intensity comparison of UCL-211 uptake in NHM, HDF and HEK (corresponding to miR211-5p conjugated with Cy5). d. Uptake of UCLs in NHM, HDF and HEK-FITC intensity (from F-DOTAP in UCLs). e. Uptake of UCLs in NHM, HDF and HEK-APC intensity (from Cy5 conjugated on miR211-5p). f. Pigmentation effect on uptake in light-pigmented (LP), medium pigmented (MP), and dark-pigmented (DP) melanocytes cultured in <t>HMGS2</t> media. Data are represented as mean ± SD. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns-not significant). Biological replicates ( N = 2) and technical replicates ( n = 3).
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    CCK-8 assay confirmed UCL, UCL-NC, and UCL-211 were not cytotoxic to NHM (a) and HDF (b) . c. Histogram for APC intensity comparison of UCL-211 uptake in NHM, HDF and HEK (corresponding to miR211-5p conjugated with Cy5). d. Uptake of UCLs in NHM, HDF and HEK-FITC intensity (from F-DOTAP in UCLs). e. Uptake of UCLs in NHM, HDF and HEK-APC intensity (from Cy5 conjugated on miR211-5p). f. Pigmentation effect on uptake in light-pigmented (LP), medium pigmented (MP), and dark-pigmented (DP) melanocytes cultured in <t>HMGS2</t> media. Data are represented as mean ± SD. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns-not significant). Biological replicates ( N = 2) and technical replicates ( n = 3).
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    CCK-8 assay confirmed UCL, UCL-NC, and UCL-211 were not cytotoxic to NHM (a) and HDF (b) . c. Histogram for APC intensity comparison of UCL-211 uptake in NHM, HDF and HEK (corresponding to miR211-5p conjugated with Cy5). d. Uptake of UCLs in NHM, HDF and HEK-FITC intensity (from F-DOTAP in UCLs). e. Uptake of UCLs in NHM, HDF and HEK-APC intensity (from Cy5 conjugated on miR211-5p). f. Pigmentation effect on uptake in light-pigmented (LP), medium pigmented (MP), and dark-pigmented (DP) melanocytes cultured in <t>HMGS2</t> media. Data are represented as mean ± SD. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns-not significant). Biological replicates ( N = 2) and technical replicates ( n = 3).
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    CCK-8 assay confirmed UCL, UCL-NC, and UCL-211 were not cytotoxic to NHM (a) and HDF (b) . c. Histogram for APC intensity comparison of UCL-211 uptake in NHM, HDF and HEK (corresponding to miR211-5p conjugated with Cy5). d. Uptake of UCLs in NHM, HDF and HEK-FITC intensity (from F-DOTAP in UCLs). e. Uptake of UCLs in NHM, HDF and HEK-APC intensity (from Cy5 conjugated on miR211-5p). f. Pigmentation effect on uptake in light-pigmented (LP), medium pigmented (MP), and dark-pigmented (DP) melanocytes cultured in <t>HMGS2</t> media. Data are represented as mean ± SD. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns-not significant). Biological replicates ( N = 2) and technical replicates ( n = 3).
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    CCK-8 assay confirmed UCL, UCL-NC, and UCL-211 were not cytotoxic to NHM (a) and HDF (b) . c. Histogram for APC intensity comparison of UCL-211 uptake in NHM, HDF and HEK (corresponding to miR211-5p conjugated with Cy5). d. Uptake of UCLs in NHM, HDF and HEK-FITC intensity (from F-DOTAP in UCLs). e. Uptake of UCLs in NHM, HDF and HEK-APC intensity (from Cy5 conjugated on miR211-5p). f. Pigmentation effect on uptake in light-pigmented (LP), medium pigmented (MP), and dark-pigmented (DP) melanocytes cultured in <t>HMGS2</t> media. Data are represented as mean ± SD. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns-not significant). Biological replicates ( N = 2) and technical replicates ( n = 3).
    Pma Free Human Melanocyte Growth Supplement (Hmgs 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CCK-8 assay confirmed UCL, UCL-NC, and UCL-211 were not cytotoxic to NHM (a) and HDF (b) . c. Histogram for APC intensity comparison of UCL-211 uptake in NHM, HDF and HEK (corresponding to miR211-5p conjugated with Cy5). d. Uptake of UCLs in NHM, HDF and HEK-FITC intensity (from F-DOTAP in UCLs). e. Uptake of UCLs in NHM, HDF and HEK-APC intensity (from Cy5 conjugated on miR211-5p). f. Pigmentation effect on uptake in light-pigmented (LP), medium pigmented (MP), and dark-pigmented (DP) melanocytes cultured in HMGS2 media. Data are represented as mean ± SD. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns-not significant). Biological replicates ( N = 2) and technical replicates ( n = 3).

    Journal: bioRxiv

    Article Title: Transdermal Delivery of Ultradeformable Cationic Liposomes Complexed with miR211-5p (UCL-211) Stabilizes BRAFV600E+ Melanocytic Nevi

    doi: 10.1101/2024.10.17.618694

    Figure Lengend Snippet: CCK-8 assay confirmed UCL, UCL-NC, and UCL-211 were not cytotoxic to NHM (a) and HDF (b) . c. Histogram for APC intensity comparison of UCL-211 uptake in NHM, HDF and HEK (corresponding to miR211-5p conjugated with Cy5). d. Uptake of UCLs in NHM, HDF and HEK-FITC intensity (from F-DOTAP in UCLs). e. Uptake of UCLs in NHM, HDF and HEK-APC intensity (from Cy5 conjugated on miR211-5p). f. Pigmentation effect on uptake in light-pigmented (LP), medium pigmented (MP), and dark-pigmented (DP) melanocytes cultured in HMGS2 media. Data are represented as mean ± SD. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns-not significant). Biological replicates ( N = 2) and technical replicates ( n = 3).

    Article Snippet: NHMs, and medium and dark pigmented melanocytes were grown in Gibco™ Medium 254 (Gibco™, US) supplemented with Gibco™ Human Melanocyte Growth Supplement-2 (HMGS-2; Gibco™, US-referred to as HMGS2 media) or Gibco™ Human Melanocyte Growth Supplement (HMGS; Gibco™ US-referred to as HMGS media).

    Techniques: CCK-8 Assay, Comparison, Cell Culture

    Quantification of miR211-5p using Abs q-PCR. a. Concentration in copies/µL of miR211-5p in the total sample confirmed to be the highest levels in the UCL-211 treated group. b. Concentration in copies/µL of miR16-5p in the total sample. c. Live cell images from fluorescent protein-based miR211-5p sponge reporter assay with cells treated with HMGS2 (control), UCL-NC, HMGS and UCL-211 at 0h and 72h. d. Overall increase in the mCherry signal after 72 h and reduction in the GFP signal (calculated as GFP/mCherry ratio) (n=3). Data are represented as mean ± SD. (* p < 0.05; **** p < 0.0001; ns-not significant).

    Journal: bioRxiv

    Article Title: Transdermal Delivery of Ultradeformable Cationic Liposomes Complexed with miR211-5p (UCL-211) Stabilizes BRAFV600E+ Melanocytic Nevi

    doi: 10.1101/2024.10.17.618694

    Figure Lengend Snippet: Quantification of miR211-5p using Abs q-PCR. a. Concentration in copies/µL of miR211-5p in the total sample confirmed to be the highest levels in the UCL-211 treated group. b. Concentration in copies/µL of miR16-5p in the total sample. c. Live cell images from fluorescent protein-based miR211-5p sponge reporter assay with cells treated with HMGS2 (control), UCL-NC, HMGS and UCL-211 at 0h and 72h. d. Overall increase in the mCherry signal after 72 h and reduction in the GFP signal (calculated as GFP/mCherry ratio) (n=3). Data are represented as mean ± SD. (* p < 0.05; **** p < 0.0001; ns-not significant).

    Article Snippet: NHMs, and medium and dark pigmented melanocytes were grown in Gibco™ Medium 254 (Gibco™, US) supplemented with Gibco™ Human Melanocyte Growth Supplement-2 (HMGS-2; Gibco™, US-referred to as HMGS2 media) or Gibco™ Human Melanocyte Growth Supplement (HMGS; Gibco™ US-referred to as HMGS media).

    Techniques: Concentration Assay, Reporter Assay, Control

    Cells at time 0 h-HMGS ( a ), UCL-NC ( c ), UCL-211 ( e ) and HMGS2 ( g ). After 72 h treatment with HMGS media (positive control) (b) and UCL-211 (f) , cells became elongated due to the presence of miR211-5p. Cells treated with HMGS2 media (negative control) (d), and UCL-NC (h) were rounded in shape with no change. Integrated analysis for population morphology using Livecyte® Analyse software over 72 h confirmed the similarity between UCL-211 and HMGS conditions, including median cell thickness (i) , median cell sphericity (j) , and median cell area (k) .

    Journal: bioRxiv

    Article Title: Transdermal Delivery of Ultradeformable Cationic Liposomes Complexed with miR211-5p (UCL-211) Stabilizes BRAFV600E+ Melanocytic Nevi

    doi: 10.1101/2024.10.17.618694

    Figure Lengend Snippet: Cells at time 0 h-HMGS ( a ), UCL-NC ( c ), UCL-211 ( e ) and HMGS2 ( g ). After 72 h treatment with HMGS media (positive control) (b) and UCL-211 (f) , cells became elongated due to the presence of miR211-5p. Cells treated with HMGS2 media (negative control) (d), and UCL-NC (h) were rounded in shape with no change. Integrated analysis for population morphology using Livecyte® Analyse software over 72 h confirmed the similarity between UCL-211 and HMGS conditions, including median cell thickness (i) , median cell sphericity (j) , and median cell area (k) .

    Article Snippet: NHMs, and medium and dark pigmented melanocytes were grown in Gibco™ Medium 254 (Gibco™, US) supplemented with Gibco™ Human Melanocyte Growth Supplement-2 (HMGS-2; Gibco™, US-referred to as HMGS2 media) or Gibco™ Human Melanocyte Growth Supplement (HMGS; Gibco™ US-referred to as HMGS media).

    Techniques: Positive Control, Negative Control, Software